A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described. The efficiency and the stability of the immobilized enzyme were demonstrated during the asymmetric reduction of a range of ketones. The cascade two-step chemo-enzymatic preparation of chiral epoxides was possible through the initial ketone bioreduction to an intermediate halohydrin followed by its intramolecular cyclization.
Rapid, selective and stable HaloTag-Lb ADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides / Dobber, J.; Pohl, M.; Ley, S. V.; Musio, B.. - In: REACTION CHEMISTRY & ENGINEERING. - ISSN 2058-9883. - 3:1(2018), pp. 8-12. [10.1039/c7re00173h]
Rapid, selective and stable HaloTag-Lb ADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides
Musio B.
2018-01-01
Abstract
A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described. The efficiency and the stability of the immobilized enzyme were demonstrated during the asymmetric reduction of a range of ketones. The cascade two-step chemo-enzymatic preparation of chiral epoxides was possible through the initial ketone bioreduction to an intermediate halohydrin followed by its intramolecular cyclization.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
