Nowadays most of the protocols used in biotechnology can be fully simulated and checked in-silico before being carried out in-vitro. Given the high level of complexity of the molecular phenomena involved in such protocols it has been observed that a consistent amount of money can be saved by both designing and simulating strategies like in-silico cloning that minimizes the probability of man-made error to occur. This aspect became especially relevant with the advent of disciplines like synthetic biology strongly relying on molecular biology protocols. Here we propose a new software package intended to provide molecular and synthetic biologists with an off-the-shelf tool to simulate widely used protocols in everyday lab practice. Here we describe routines meant to assist researchers through the whole experiment: from the cloning strategy design to the alignment of predicted and actual results. Cues for further research in this field are provided in the final part of the manuscript.
A Novel Tool for Assisted In-silico Cloning and Sequence Editing in Molecular Biology / Bevilacqua, V.; Menolascina, F.; Aurora, D.; Lucivero, S.; Quatela, N. F. - In: Advanced Intelligent Computing Theories and Applications / [a cura di] Huang, DS; McGinnity, M; Heutte, L; Zhang, XP. - STAMPA. - Berlin : Springer, 2010. - ISBN 978-3-642-14477-6. - pp. 239-245 [10.1007/978-3-642-14831-6_32]
A Novel Tool for Assisted In-silico Cloning and Sequence Editing in Molecular Biology
Bevilacqua, V.;
2010-01-01
Abstract
Nowadays most of the protocols used in biotechnology can be fully simulated and checked in-silico before being carried out in-vitro. Given the high level of complexity of the molecular phenomena involved in such protocols it has been observed that a consistent amount of money can be saved by both designing and simulating strategies like in-silico cloning that minimizes the probability of man-made error to occur. This aspect became especially relevant with the advent of disciplines like synthetic biology strongly relying on molecular biology protocols. Here we propose a new software package intended to provide molecular and synthetic biologists with an off-the-shelf tool to simulate widely used protocols in everyday lab practice. Here we describe routines meant to assist researchers through the whole experiment: from the cloning strategy design to the alignment of predicted and actual results. Cues for further research in this field are provided in the final part of the manuscript.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.