The main purpose of the paper is to assess the reliability of particular configuration of Laser Doppler Vibrometry intended to be suitable for the analysis of vibrations affecting rotating components of machines. In the paper an analysis of uncertainty has been carried out due to the possible static misalignments of the mirrors utilized in the experimental apparatus set up for the estimation of the out of plane vibrations of moving (rotating) objects. Those misalignments have to be distinguished from the so called dynamic ones due instead to dynamic effects induced by the movement of the object itself. That is in order to give a better characterization of the self-tracking technique employed with the use of a 1D LDV and of the measurements done by means of it, whose uncertainties are mostly linked to the interaction between environment and instrumentation.