In this work, a Coarse-Grained Lattice Spring Model to characterize the mechanical behavior of human mesenchymal stem cells subjected to nanoindentation measurements is presented. The model simulated the action of adhesive structures acting on cells, necessary for attaching them to a substrate, and a nanoindentation process, performed by means of an atomic force microscope with a spherical tip. Cells were hypothesized to behave as elastic materials and the model included several subcellular components such as cell cortex and cytoskeleton. The lattice spring model was integrated within an optimization algorithm that iteratively compared the force-indentation curve numerically predicted to the data experimentally obtained, until a best fit condition was reached. The computed mechanical properties of the cell were compared to those obtained via the Hertz contact theory and finite element modelling, showing a good agreement. The proposed lattice spring model appears as a promising tool that can be used, with a very low computational cost, to characterize cell materials and other biological materials.

A Coarse-Grained Lattice Spring Model to Characterize Nanoindented Stem Cells

Vaiani L.
;
Fiorentino M.;Gattullo M.;Manghisi V. M.;Uva A. E.;Boccaccio A.
2022

Abstract

In this work, a Coarse-Grained Lattice Spring Model to characterize the mechanical behavior of human mesenchymal stem cells subjected to nanoindentation measurements is presented. The model simulated the action of adhesive structures acting on cells, necessary for attaching them to a substrate, and a nanoindentation process, performed by means of an atomic force microscope with a spherical tip. Cells were hypothesized to behave as elastic materials and the model included several subcellular components such as cell cortex and cytoskeleton. The lattice spring model was integrated within an optimization algorithm that iteratively compared the force-indentation curve numerically predicted to the data experimentally obtained, until a best fit condition was reached. The computed mechanical properties of the cell were compared to those obtained via the Hertz contact theory and finite element modelling, showing a good agreement. The proposed lattice spring model appears as a promising tool that can be used, with a very low computational cost, to characterize cell materials and other biological materials.
Lecture Notes in Mechanical Engineering
978-3-030-91233-8
978-3-030-91234-5
Springer Science and Business Media Deutschland GmbH
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11589/236400
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