In this work a hydrogel, based on a blend of two gellan gums with different acyl content embedding lignin (up to 0.4%w/v) and crosslinked with magnesium ions, was developed for cartilage regeneration. The physico-chemical characterizations established that no chemical interaction between lignin and polysaccharides was detected. Lignin achieved up to 80 % of ascorbic acid's radical scavenging activity in vitro on DPPH and ABTS radicals. Viability of hMSC onto hydrogel containing lignin resulted comparable to the lignin-free one (>70 % viable cells, p > 0.05). The presence of lignin improved the hMSC 3D-constructs chondrogenesis, bringing to a significant (p < 0.05) up-regulation of the collagen type II, aggrecan and SOX 9 chondrogenic genes, and conferred bacteriostatic properties to the hydrogel, reducing the proliferation of S. aureus and S. epidermidis. Finally, cellularized 3D-constructs were manufactured via 3D-bioprinting confirming the processability of the formulation as a bioink and its unique biological features for creating a physiological milieu for cell growth.

A bioprintable gellan gum/lignin hydrogel: A smart and sustainable route for cartilage regeneration / Bonifacio, Maria A; Cometa, Stefania; Cochis, Andrea; Scalzone, Annachiara; Gentile, Piergiorgio; Scalia, Alessandro C; Rimondini, Lia; Mastrorilli, Piero; De Giglio, Elvira. - In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. - ISSN 0141-8130. - STAMPA. - 216:(2022), pp. 336-346. [10.1016/j.ijbiomac.2022.07.002]

A bioprintable gellan gum/lignin hydrogel: A smart and sustainable route for cartilage regeneration

Mastrorilli, Piero;
2022-01-01

Abstract

In this work a hydrogel, based on a blend of two gellan gums with different acyl content embedding lignin (up to 0.4%w/v) and crosslinked with magnesium ions, was developed for cartilage regeneration. The physico-chemical characterizations established that no chemical interaction between lignin and polysaccharides was detected. Lignin achieved up to 80 % of ascorbic acid's radical scavenging activity in vitro on DPPH and ABTS radicals. Viability of hMSC onto hydrogel containing lignin resulted comparable to the lignin-free one (>70 % viable cells, p > 0.05). The presence of lignin improved the hMSC 3D-constructs chondrogenesis, bringing to a significant (p < 0.05) up-regulation of the collagen type II, aggrecan and SOX 9 chondrogenic genes, and conferred bacteriostatic properties to the hydrogel, reducing the proliferation of S. aureus and S. epidermidis. Finally, cellularized 3D-constructs were manufactured via 3D-bioprinting confirming the processability of the formulation as a bioink and its unique biological features for creating a physiological milieu for cell growth.
2022
A bioprintable gellan gum/lignin hydrogel: A smart and sustainable route for cartilage regeneration / Bonifacio, Maria A; Cometa, Stefania; Cochis, Andrea; Scalzone, Annachiara; Gentile, Piergiorgio; Scalia, Alessandro C; Rimondini, Lia; Mastrorilli, Piero; De Giglio, Elvira. - In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. - ISSN 0141-8130. - STAMPA. - 216:(2022), pp. 336-346. [10.1016/j.ijbiomac.2022.07.002]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11589/241060
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