The present invention relates to a method for the design of oligonucleotides suitable for PCR, Real time PCR, microarrays and, more generally for DNA and RNA amplification and/or detection, said method allowing a quick and simultaneous computation of more parameters, and computer programs capable of carrying out the method of the invention. The present invention discloses a method and a computer program carrying out said method integrating, all in one, a high functionality, and a number of parameters that are not measured together in the state of the art, for more than one oligonucleotide spamming over a single input sequence starting from sequences in FASTA format and giving a few input data such as threshold temperatures, oligonucleotide length and sampling frequencies.
METHOD FOR THE DESIGN AND ENGINEERING OF OLIGONUCLEOTIDES / Paradiso, Angelo; Tommasi, Stefania; Menolascina, Filippo; Monaco, Alessandro; Bevilacqua, Vitoantonio; Mastronardi, Giuseppe. - (2007).
METHOD FOR THE DESIGN AND ENGINEERING OF OLIGONUCLEOTIDES
BEVILACQUA, Vitoantonio;MASTRONARDI, Giuseppe
2007-01-01
Abstract
The present invention relates to a method for the design of oligonucleotides suitable for PCR, Real time PCR, microarrays and, more generally for DNA and RNA amplification and/or detection, said method allowing a quick and simultaneous computation of more parameters, and computer programs capable of carrying out the method of the invention. The present invention discloses a method and a computer program carrying out said method integrating, all in one, a high functionality, and a number of parameters that are not measured together in the state of the art, for more than one oligonucleotide spamming over a single input sequence starting from sequences in FASTA format and giving a few input data such as threshold temperatures, oligonucleotide length and sampling frequencies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
