According to the World Health Organization (WHO) forecasts, AntiMicrobial Resistance (AMR) will represent the leading cause of death worldwide in the next decades. To prevent this phenomenon, rapid antimicrobial susceptibility testing is needed to guide the choice of the proper antibiotic. In this context, we propose a chip-scale system, mainly based on a microfluidic channel combined with a pattern of engineered electrodes, to efficiently test an antibiotic on a bacteria sample. The use of dielectrophoretic (DEP) forces enable the sorting of live/dead bacteria, such as Escherichia Coli, with an efficiency larger than 99% for rapid monitoring of the antimicrobial susceptibility at the single-bacterium level.
Sorting of Live/dead Escherichia Coli by Means of Dielectrophoresis for Rapid Antimicrobial Susceptibility Testing / di Toma, A.; Brunetti, G.; Sasanelli, N.; Armenise, M. N.; Ciminelli, C.. - ELETTRONICO. - 1036:(2023), pp. 196-202. [10.1007/978-3-031-30333-3_25]
Sorting of Live/dead Escherichia Coli by Means of Dielectrophoresis for Rapid Antimicrobial Susceptibility Testing
di Toma, A.;Brunetti, G.;Sasanelli, N.;Armenise, M. N.;Ciminelli, C.
2023-01-01
Abstract
According to the World Health Organization (WHO) forecasts, AntiMicrobial Resistance (AMR) will represent the leading cause of death worldwide in the next decades. To prevent this phenomenon, rapid antimicrobial susceptibility testing is needed to guide the choice of the proper antibiotic. In this context, we propose a chip-scale system, mainly based on a microfluidic channel combined with a pattern of engineered electrodes, to efficiently test an antibiotic on a bacteria sample. The use of dielectrophoretic (DEP) forces enable the sorting of live/dead bacteria, such as Escherichia Coli, with an efficiency larger than 99% for rapid monitoring of the antimicrobial susceptibility at the single-bacterium level.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
